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Library preparation

This guide summarizes library preparation for sequencing analysed with ROBIN.

Overview

ROBIN is designed for Oxford Nanopore (ONT) libraries. For CNS tumour work you can use either:

  1. The ROBIN-specific protocol on protocols.io, or
  2. The standard ONT LSK114 ligation workflow.

Input DNA

Adaptive sampling works best with read lengths around 8–12 kb; follow your chosen protocol to hit that range.

Options

ROBIN protocol

ROBIN: A Unified Nanopore-based Sequencing Assay — fragmentation, ligation, QC, and troubleshooting tuned for this use case.

Standard ONT LSK114

ONT LSK114 product / documentation and library preparation hub.

DNA input

  • High-quality DNA (e.g. A260/A280 ~1.8–2.0)
  • Minimum ~100 ng; optimal often 500 ng–1 µg
  • Elute in TE or nuclease-free water

Next steps

  1. Configure MinKNOW for your run — MinKNOW configuration
  2. Start ROBIN — Quickstart

Resources